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PCR strategies / edited by Michael A. Innis, David H. Gelfand and John J. Sninsky.

Contributor(s): Material type: TextTextPublication details: San Diego ; London : Academic Press, c1995.Description: xv,373p. : ill. ; 25cmISBN:
  • 9780123721822 (cased) :
  • 9780123721839 (pbk)
  • 0123721830 (paper : acid-free paper)
Other title:
  • Polymerase chain reaction strategies
Subject(s): DDC classification:
  • 574.873282 INN
Contents:
1.The use of cosolvents to enhance amplification by the polymerase chain reaction -- 2.DNA polymerase fidelity: misinsertions and mismatched extensions -- 3.Extraction of nucleic acids: sample preparation -- 4.Thermostable DNA polymerases -- 5.Amplifaction of RNA: high temperature reverse transcription and DNA amplification with Thermus thermophilus DNA polymerase -- 6.Nucleic acid hydridization and unconventional bases -- 7.Practical considerations for the design of quantitative PCD assays -- Part 2.Analysis of PCD products -- 8.Carrier dection of cystic fibrosis mutations using PCD-amplified DNA and a mismatch-binding protein, MutS -- 9.Single-stranded conformational polymorphisms -- 10.Analysis of PCR products by covalent reverse dot blot hybridization -- 11.High-performance liquid chormoatograhy analysis of PCR products -- 12.Heteroduplex mobility assays for phylogenetic analysis -- 13.PCR amplifaction of VNTRs -- Part three. Research applications -- 14.Site-specific mutagenesis uing the polymerase chain reaction -- 15.Exact quantification of DNA-RNA copy numbers by PCR-TGGE -- 16.the in site PCR: amplification and detection of DNA in a cellular context -- 17.Y chromosome-specific PCR: maternal blood -- 18.Genomic subtraction -- 19.DNA amplification-restricted transcription tranlsation [DARTT]: analysis of in vitro and in situ protein functions and intermolecular assembly -- 20.DNA and RNA fingerprinting using arbitrarily primed PCR -- 21.PCR-based screening of yeast artificial chromosome libraries -- 22.Oligonucleotide ligans that discriminate between theophylline and caffeine -- 23.Generation of single-chain antioboyd fragments by PCR -- 23.Generation of single chain antibody fragments by PCR -- 24.Longer PCR amplifications -- 25.Direct analysis of specific bonds from arbitrarily primed PCR reactions -- Part four.Alternative amplificaton strategies -- 26.Detection of Leber's hereditary optic neuropathy by nonradioactive chain reaction -- 27.Detection of Listeria monocytogenes by PCR-coupled ligase chain reaction.
Summary: PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a set of up-to-date strategies and protocols for using PCR in all relevant research areas.
Holdings
Item type Current library Call number Copy number Status Date due Barcode
Long Loan TUS: Midlands, Main Library Athlone General Lending 574.873282 INN (Browse shelf(Opens below)) 1 Available 107993

Includes index.

Includes bibliographical references and index.

1.The use of cosolvents to enhance amplification by the polymerase chain reaction -- 2.DNA polymerase fidelity: misinsertions and mismatched extensions -- 3.Extraction of nucleic acids: sample preparation -- 4.Thermostable DNA polymerases -- 5.Amplifaction of RNA: high temperature reverse transcription and DNA amplification with Thermus thermophilus DNA polymerase -- 6.Nucleic acid hydridization and unconventional bases -- 7.Practical considerations for the design of quantitative PCD assays -- Part 2.Analysis of PCD products -- 8.Carrier dection of cystic fibrosis mutations using PCD-amplified DNA and a mismatch-binding protein, MutS -- 9.Single-stranded conformational polymorphisms -- 10.Analysis of PCR products by covalent reverse dot blot hybridization -- 11.High-performance liquid chormoatograhy analysis of PCR products -- 12.Heteroduplex mobility assays for phylogenetic analysis -- 13.PCR amplifaction of VNTRs -- Part three. Research applications -- 14.Site-specific mutagenesis uing the polymerase chain reaction -- 15.Exact quantification of DNA-RNA copy numbers by PCR-TGGE -- 16.the in site PCR: amplification and detection of DNA in a cellular context -- 17.Y chromosome-specific PCR: maternal blood -- 18.Genomic subtraction -- 19.DNA amplification-restricted transcription tranlsation [DARTT]: analysis of in vitro and in situ protein functions and intermolecular assembly -- 20.DNA and RNA fingerprinting using arbitrarily primed PCR -- 21.PCR-based screening of yeast artificial chromosome libraries -- 22.Oligonucleotide ligans that discriminate between theophylline and caffeine -- 23.Generation of single-chain antioboyd fragments by PCR -- 23.Generation of single chain antibody fragments by PCR -- 24.Longer PCR amplifications -- 25.Direct analysis of specific bonds from arbitrarily primed PCR reactions -- Part four.Alternative amplificaton strategies -- 26.Detection of Leber's hereditary optic neuropathy by nonradioactive chain reaction -- 27.Detection of Listeria monocytogenes by PCR-coupled ligase chain reaction.

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a set of up-to-date strategies and protocols for using PCR in all relevant research areas.

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