Molecular toxicology protocols / edited by Phouthone Keohavong and Stephen G. Grant.

Originally published: 2005.

Includes bibliographical references and index.

21.Analysis of K-RAS and P53 mutations in sputum samples. Weimin Gao and Phouthone Keohavong -- 22.Allele-specific competitive blocker-PCR detection of rare base substitution. Barbara L. Parsons, Page B. McKinzie, and Robert H. Heilich -- 23.Gel-based nonradioiactive single-strand conformational polymorphism and mutation detection: limitations and solutions. Vibhuti Gupta ... et al -- 24.Detection and characterization of oncogene mutations in preneloplastic and early neoplstic lesions. Toshinari Minamoto -- 25.Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated PCR and inverse PCR. Michael J. Villalobos, Christopher J. Betti, and Andrew T. M. Vaughan -- Part V: Analysis of DNA repair mechanisms -- 26.Microsatellite instability: an indirect assay to detect defects in the cellular mismatch repair machinery. Anjana Saha. Harendra K. Bairwa, and Ramesh Bamezai -- 29.An immunoassay for measuring repair of ultraviolet photoproducts. Shirley McCready -- 30.Analysis of DNA double-strand break repair by nonhomologous end joining in cell-free extracts from mammalian cells. Petra Pfeiffer ... et al -- 31.Measuring recombination proficiency in mouse embryonic stem cells. Andrew J. Pierce andMaria Jasin -- Part VI: Array technologies -- 32.Strategies for measurement of biotransformaton enzyme gene expression. Marjorie Romkes and Shama C. Buch -- 33.Genotyping technologies: application to biotransformation enzyme genetic polymorphism screening. Marjorie Romkes and Shama C. Buch -- 34.TaqMan fluorogenic detection system to analyze gene transcription in autopsy material. Laori Shintani-Ishida, Bao-Li Xhu and Hitoshi Maeda -- 35.Development of quantitative reverse transcriptase PCR assays for measuring gene expression. Tony E. Godfrey and Lori A. Kelly -- Part VII: Apoptosis -- 36.Quantification of selective phosphatidylserine oxidation during apoptosis. James P. Fabisiak ... [et al] -- 37.Quantitative method of measuring phosphatidyloserine externalization during apoptosis using electron paramagnetic resoance spectroscopy and annexin-conjugated iron. James P. Fabisiak ... [et al] 38.Detection of programme cell death in cells exposed to genotoxic agents using a capase activation assay. Micahel E. Gehring and Patrick P. Koty.

Part I: Analysis of DNA adducts -- 1.P-Postlabeling analysis of DNA adducts. David H. Phillips, Alan Hewer, and Volker M. Arlt -- 2.Modification of the P-Postlabing method to detect a single adduct species as a single spot. Masako Ochiai, Takashi Sugimura, and Minako Nagao -- 3.DNA isolation and sample preparation for quantification of adduct levels by accelerator mass spectrometry. Karen H. Dingley et al -- 4.Fluoroimaging-based immunoassay of DNA photoproducts in ultraviolet-B-irradiated tadpoles. Iovanna Pandelova, Stephen R. Hewitt, and John B. Hays -- 5.Analysis of DNA Strand Cleavage at abasic sites. Walter A. Deutsch and Vijay Hegde -- Part II.Detection of chromosomal and genome-wide damage -- 6.Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes. Pataje G. S, Prasanna and William F. Blakely -- 7.Mutagen-induced chromatid breakage as a marker of cancer risk. Xifeng Wu, Yun-Ling Zheng and T. C. Hsu -- 8.Flow cytometric analysis of micronuclei in erythrocytes. Jan Graweþ -- 10.Computerized image analysis software for the comet assay. R. C. Chaubey -- 11.The comet-FSH technique: a tool for detection of specific DNA damage and reapair. Alexander Rapp, Michael Hausmann, and Karl Otto Greulich -- 12.DNA double strand break damage and repair assessed by pulsed-field gel electrophoresis.

Part II.Detection and characterization of surrogate gene mutation -- 13.Analysis of in vivo mutation in the Hprt and Tk gense of mouse lympocytes. Vasily N. Dobrovolsky, Josephy G. Shaddock, and Robert H. Heflich -- 14.Quantifiying in vivo somatic mutations using trasngenic mouse models. Roy R. Swiger -- 15.Methods for detecting someatic mutations in vitro: the human t-cell cloning assay selecting for HPRT mutants.Sai-Mei Hou -- 16.Molecular analysis of mutations in the human HRPT gene. Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant -- 17.Simultanesou quantification of the t(14;18) and HPRT Exon 2/3 delections in human lymphocytes. James C. Fuscoe -- 18.The GPA in vivo somatic mutation assay. Stephen C. Grant -- 19.Flow cytometric measurement of mutant t cells with altered expression of TCR: detecting somatic mutations in humans and mice. Seishi Kyoizumi Kusunoki, and Tomonori Hayashi -- Part IV: Detection and characterization of cancer gene mutation -- 20.Mutation screeing of the TP53 gene by temporal temperature gradient gel electrophoresis. Therese Sorlie ... et al

'Molecular Toxicology Protocols' brings together the methods most commonly used to elucidate specific molecular aspects of toxicology.