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Biochemistry in the lab a manual for undergraduates/ by Benjamin F. Lasseter

By: Material type: TextTextPublisher: Boca Raton London New York CRC Press, Taylor & Francis Group 2020Copyright date: 2020Description: xiii, 187 pages, Illustrated, 24 cmISBN:
  • 9781138589964
  • 1138589969
  • 9781138589926
  • 1138589926
Subject(s): Additional physical formats: No titleDDC classification:
  • 572.078 LAS
Contents:
1.Buffers. 2.Assays. 3.Protein concentration. 4.ELISA. 5.Salting out proteins and other biomolecules. 6.A discussion of isoelectric point and effective charge. 7.Column chromatography. 8.Michaelis-Menten Kinetics. 9.Protein purification. 10.Polyacrylamide gels. 11.In Silco biochemistry: the evolution of Globins. 12.Growing crystals of hemoglobin. 13.Enzyme inhibition. 14.Multisubstrate kinetics. 15.Fluorescence and denaturation. 16.Fluorescence studies of ligand binding. 17.DNA restriction digests. 18.Western blotting.
Summary: "Biochemistry in the Lab: A Manual for Undergraduates presents a comprehensive approach to modern biochemistry laboratory teaching together with a complete experimental experience, from molecular biology, cloning, and protein expression, to purification and characterization"-
Holdings
Item type Current library Call number Status Date due Barcode
Short Loan TUS: Midlands, Main Library Athlone General Lending 572.078 LAS (Browse shelf(Opens below)) Available 225842
Two Hour Loan TUS: Midlands, Main Library Athlone General Lending 572.078 LAS (Browse shelf(Opens below)) Available 225715

Includes index.

1.Buffers. 2.Assays. 3.Protein concentration. 4.ELISA. 5.Salting out proteins and other biomolecules. 6.A discussion of isoelectric point and effective charge. 7.Column chromatography. 8.Michaelis-Menten Kinetics. 9.Protein purification. 10.Polyacrylamide gels. 11.In Silco biochemistry: the evolution of Globins. 12.Growing crystals of hemoglobin. 13.Enzyme inhibition. 14.Multisubstrate kinetics. 15.Fluorescence and denaturation. 16.Fluorescence studies of ligand binding. 17.DNA restriction digests. 18.Western blotting.

"Biochemistry in the Lab: A Manual for Undergraduates presents a comprehensive approach to modern biochemistry laboratory teaching together with a complete experimental experience, from molecular biology, cloning, and protein expression, to purification and characterization"-

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