gogo
Amazon cover image
Image from Amazon.com

Advanced methods in molecular biology and biotechnology [electronic resource] a practical lab manual / Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool.

By: Contributor(s): Material type: TextTextPublication details: London : Academic Press, [2021].Description: 1 online resourceISBN:
  • 9780128244500
  • 012824450X
Subject(s): Genre/Form: Additional physical formats: Print version:: No title; Print version:: No titleOnline resources:
Contents:
Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table
Materials and equipments -- Materials and equipments required -- Reagents required -- Step-by-step method details -- Timing: 1-1.5 h (depending on the size of the sample) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 3 SDS-polyacrylamide gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details
Timing: 3-4 h (depends on the plate size) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 4 Immunoelectrophoresis -- Definition -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards
Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- References -- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution.
Summary: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
Holdings
Item type Current library Call number Status Date due Barcode
Ebook TUS: Midlands, Main Library Athlone Online Available

Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table

Materials and equipments -- Materials and equipments required -- Reagents required -- Step-by-step method details -- Timing: 1-1.5 h (depending on the size of the sample) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 3 SDS-polyacrylamide gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details

Timing: 3-4 h (depends on the plate size) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 4 Immunoelectrophoresis -- Definition -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards

Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- References -- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution.

Restricted to GMIT Students and Staff

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

Powered by Koha