Kenzan method for scaffold-Free biofabrication edited by Koichi Nakayama.

Development of a scaffold-free 3D biofabrication system "Kenzan method". Koichi Nakayama Position of the Kenzan method in the space-time of tissue engineering. Nicanor I. Moldovan Development of the Bio 3D printer "Regenova", the robotic system. Masahiro Sakamoto and Kenji Yoneda Scaffold-free biofabrication for articular cartilage (and subchondral bone). Daiki Murata Scaffold-free biofabrication of liver. Yusuke Yanagi, Thoshiharu Matsuura, and Tomoaki Taguchi Artificial trachea: past, present, and future. Keitaro Matsumoto and Takeshi Nagayasu Cardiac tissue creation with the Kenzan method. Hiroshi Matshushita, Vivian Nguyen, Katherine Nurminsky, and Narutoshi Hibino Scaffold-free autologous cell-based vascular graft for clinical applications. Manabu Itoh Peripheral nerve regeneration using Bio 3D never conduits. Ryosuke Iteguchi, Tomoki Ayoama, Hirofumi Yurie et al Regeneration of the diaphragm. Yusuke Yanagi, Xiu-Ying Zhang, Kouji Nagata et al Bio-3D printed organs as drug testing tools. Kenichi Arai and Koichi Kakayama Biomaterial-related surgical site infection: anti-infectious metal coating on biomaterials. Hironobu Koseki and Shiro Kajiyama

This is the first book about the "Kenzan" method for scaffold-free biofabrication, which does not rely on biomaterials as scaffolds to ensure correct multicellular spheroid positioning for building three dimensional construct only made from cells. The book explains the basic principles and concepts of the microneedle-based ("Kenzan") method of building surgically-implantable tissue constructs using robotic cell spheroid-based three-dimensional bioprinting, a novel technology that opens up unique opportunities for the bioengineering of tissues and organs. First book on the novel Kenzan method of tissue engineering; Explains basic concepts and applications for organ regeneration modeling; Introduces a unique robotic system for scaffold-free cell construction.