Analytical biochemistry /
David J. Holme and Hazel Peck.
- 2nd ed.
- Harlow : Longman Scientific & Technical, 1993.
- xv,507p. : ill. ; 24cm.
Previous ed.: 1983.
Includes bibliographies and index.
(cont) Substrate assay methods -- Immobilized enzymes -- Treatment of cells and tissues for enzyme assay -- Carbohydrates -- General structure and function -- Chemical methods of carbohydrate analysis -- Enzymic methods of carbohydrate analysis -- Separation and identification of carbohydrate mixtures -- Amino acids -- General structure and properties -- General reactions -- N-terminal analysis -- Reactions of specific amino acids -- Separation of amino acid mixtures -- Amino acid analyser -- Proteins -- Protein structure -- General methods of quantitation -- Separation of proteins -- Lipids -- Fatty acids -- Simple lipids -- Complex lipids -- Lipid-protein structures -- Sample preparation and handling -- Quantitative methods -- Separation of lipid mixtures -- Nucleic acids -- Nucleic acid composition and structure -- Isolation and purification of nucleic acids -- Methods of nucleic acid analysis -- Vectors -- DNA sequencing -- Numbering and classification of enzymes. General principles of analytical biochemistry -- Selection of a valid method of analysis -- Quality of data -- Presentation of data -- Spectroscopy -- Interaction of radiation with matter -- Molecular absorptiometry -- Absorptiometer design -- Molecular fluorescence techniques -- Atomic spectroscopy techniques -- Magnetic resonance spectroscopy -- Separation methods -- Principles of separation techniques -- Methods based on polarity -- Methods based on ionic nature -- Methods based on size -- Methods based on shape -- Electroanalytical methods -- Potentiometry -- Conductimetry -- Coulometry -- Voltammetry -- Biosensors -- Radio-isotopes -- Nature of radioactivity -- Detection and measurement of radioactivity -- Biochemical uses of isotopes -- Automated methods of analysis -- Discrete analysers -- Flow analysis -- Robotics -- Immunological methods -- General processes of the immune response -- Antigen-antibody reactions -- Analytical techniques-precipitation reactions -- Analytical techniques-immunoassay -- Enzymes -- Nature of enzymes -- Monitoring techniques -- Enzyme assay methods.
Aimed primarily at undergraduate students, this text examines the analytical aspects of biochemistry and aims to provide sufficient information to enable the student to select the techniques appropriate for a particular analytical problem and develop a valid and reliable analytical method.