Short protocols in molecular biology /
Protocols in molecular biology
Frederick M. Ausubel ... [et al.].
- 4th ed.
- New York ; Chichester : Wiley, 1999.
- 1104p. : ill. ; 23 cm.
Previous ed.: 1995.
Includes bibliographical references.
Ch. 14. In situ hybridization and immunohistochemistry -- Ch. 15. The polymerase chain reaction -- Ch. 16. Protein expression -- Ch. 17. Analysis of protein phosphorylation -- Ch. 18. Sequence similarity searching using the blast family of programs -- Ch. 19. Analysis of protein interactions. Ch. 1. Escherichia coli, plasmids, and bacteriophages -- Ch. 2. Preparation and analysis of DNA -- Ch. 3. Enzymatic manipulation of DNA and RNA -- Ch. 4. Preparation and analysis of RNA -- Ch. 5. Construction of recombinant DNA libraries -- Ch. 6. Screening of recombinant DNA libraries -- Ch. 7. DNA sequencing -- Ch. 8. Mutagenesis of cloned DNA -- Ch. 9. Introduction of DNA into mammalian cells -- Ch. 10. Analysis of proteins -- Ch. 11. Immunology -- Ch. 12. DNA-protein interactions -- Ch. 13. Saccharomyces cerevisiae
This fourth edition contains over 220 protocols presented as abridged versions that provide essential core information. The text offers researchers a combined laboratory manual and single-volume reference to molecular biology technology.