DNA science : a first course.
- 2nd ed. / David A. Micklos, Greg A. Freyer with David A. Crotty.
- Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2003.
- xii, 575 p. : ill. (some col.), ports. ; 28 cm.
Previous ed.: 1992.
Includes bibliographical references and index.
1.How we learned that DNA is the genetic material -- 2.How we learned the function of DNA -- 3.How we learned how genes are regulated -- 4.Basic tools and techniques of DNA science -- 5.Methods for finding and expressing important genes -- 6.Modern methods for analyzing whole genomes -- 7.The DNA science of cancer -- 8.Applying DNA science to human genetics and evolution -- Laboratories: Lab safety and adherence to National Institute of Health Guidelines -- 1.Measurements, micropipetting, and sterile techniques -- 2.Bacterial culture techniques: A.Isolation of individual colonies -- B.Overnight suspension culture -- C.Mid-log suspension culture -- 3.DNA restriction anlaystis -- 4.Effects of DNA methylation on restriction -- 5.Rapid colony transformation of E. coli with plasmid DNA -- 6.Assay for antibiotic resistance enzyme -- 7.Purfication and identification of recombinant GFP -- A.Purification and identification of plasmid DNA -- B.PAGe anaysis of purified -- 8.Purification and identification of Plasmid DNA -- A.Plasmid minipreparation of pAMP -- B.Restriction analysis of purified pAMP -- 9.Recombination of antibiotic resistance genes -- A.Restriction digest of plasmids pAMP and pKAN -- B.Ligation of pAMP and pKan restriction fragments -- 10.Transformation of E. coli with recombinant DNA -- A.Classic procedure for preparing competent cells -- B.Transforming E. coli with recombinant DNA -- 11.Replica plating to identify mixed E. coli populations -- 12.Purification and identification of recombinant DNA -- A.Plasmid minipreparation of pAMP/pKan recombinants -- B.Restriction analysis of purified recombinant DNA.