TY  - BOOK
AU  - Keohavong,Phouthone
AU  - Keohavong,Phouthone
AU  - Grant,Stephen G.
TI  - Molecular toxicology protocols
T2  - Methods in molecular biology
SN  - 9781617373473 (pbk.) :
AV  - RA1220.3 
U1  - 615.9 KEO 
PY  - 2011///
CY  - Totowa, N.J., London
PB  - Humana, Springer [distributor]
KW  - Molecular toxicology
KW  - Laboratory manuals
KW  - Health and Wellbeing
KW  - ukslc
KW  - Pharmacology
KW  - thema
KW  - Biochemistry
N1  - Originally published: 2005; Includes bibliographical references and index; 21.Analysis of K-RAS and P53 mutations in sputum samples. Weimin Gao and Phouthone Keohavong -- 22.Allele-specific competitive blocker-PCR detection of rare base substitution. Barbara L. Parsons, Page B. McKinzie, and Robert H. Heilich -- 23.Gel-based nonradioiactive single-strand conformational polymorphism and mutation detection: limitations and solutions. Vibhuti Gupta ... et al -- 24.Detection and characterization of oncogene mutations in preneloplastic and early neoplstic lesions. Toshinari Minamoto -- 25.Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated PCR and inverse PCR. Michael J. Villalobos, Christopher J. Betti, and Andrew T. M. Vaughan -- Part V: Analysis of DNA repair mechanisms -- 26.Microsatellite instability: an indirect assay to detect defects in the cellular mismatch repair machinery. Anjana Saha. Harendra K. Bairwa, and Ramesh Bamezai -- 29.An immunoassay for measuring repair of ultraviolet photoproducts. Shirley McCready -- 30.Analysis of DNA double-strand break repair by nonhomologous end joining in cell-free extracts from mammalian cells. Petra Pfeiffer ... et al -- 31.Measuring recombination proficiency in mouse embryonic stem cells. Andrew J. Pierce andMaria Jasin -- Part VI: Array technologies -- 32.Strategies for measurement of biotransformaton enzyme gene expression. Marjorie Romkes and Shama C. Buch -- 33.Genotyping technologies: application to biotransformation enzyme genetic polymorphism screening. Marjorie Romkes and Shama C. Buch -- 34.TaqMan fluorogenic detection system to analyze gene transcription in autopsy material. Laori Shintani-Ishida, Bao-Li Xhu and Hitoshi Maeda -- 35.Development of quantitative reverse transcriptase PCR assays for measuring gene expression. Tony E. Godfrey and Lori A. Kelly -- Part VII: Apoptosis -- 36.Quantification of selective phosphatidylserine oxidation during apoptosis. James P. Fabisiak ... [et al] -- 37.Quantitative method of measuring phosphatidyloserine externalization during apoptosis using electron paramagnetic resoance spectroscopy and annexin-conjugated iron. James P. Fabisiak ... [et al]  38.Detection of programme cell death in cells exposed to genotoxic agents using a capase activation assay. Micahel E. Gehring and Patrick P. Koty; Part I: Analysis of DNA adducts -- 1.P-Postlabeling analysis of DNA adducts. David H. Phillips, Alan Hewer, and Volker M. Arlt -- 2.Modification of the P-Postlabing method to detect a single adduct species as a single spot. Masako Ochiai, Takashi Sugimura, and Minako Nagao -- 3.DNA isolation and sample preparation for quantification of adduct levels by accelerator mass spectrometry. Karen H. Dingley et al -- 4.Fluoroimaging-based immunoassay of DNA photoproducts in ultraviolet-B-irradiated tadpoles. Iovanna Pandelova, Stephen R.  Hewitt, and John B. Hays -- 5.Analysis of DNA Strand Cleavage at abasic sites. Walter A. Deutsch and Vijay Hegde -- Part II.Detection of chromosomal and genome-wide damage -- 6.Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes. Pataje G. S, Prasanna and William F. Blakely -- 7.Mutagen-induced chromatid breakage as a marker of cancer risk. Xifeng Wu, Yun-Ling Zheng and T. C. Hsu -- 8.Flow cytometric analysis of micronuclei in erythrocytes. Jan Graweþ -- 10.Computerized image analysis software for the comet assay. R. C. Chaubey -- 11.The comet-FSH technique: a tool for detection of specific DNA damage and reapair. Alexander Rapp, Michael Hausmann, and Karl Otto Greulich -- 12.DNA double strand break damage and repair assessed by pulsed-field gel electrophoresis; Part II.Detection and characterization of surrogate gene mutation -- 13.Analysis of in vivo mutation in the Hprt and Tk gense of mouse lympocytes. Vasily N. Dobrovolsky, Josephy G. Shaddock, and Robert H. Heflich -- 14.Quantifiying in vivo somatic mutations using trasngenic mouse models. Roy R. Swiger -- 15.Methods for detecting someatic mutations in vitro: the human t-cell cloning assay selecting for HPRT mutants.Sai-Mei Hou -- 16.Molecular analysis of mutations in the human HRPT gene. Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant -- 17.Simultanesou quantification of the t(14;18) and HPRT Exon 2/3 delections in human lymphocytes. James C. Fuscoe -- 18.The GPA in vivo somatic mutation assay. Stephen C. Grant -- 19.Flow cytometric measurement of mutant t cells with altered expression of TCR: detecting somatic mutations in humans and mice. Seishi Kyoizumi Kusunoki, and Tomonori Hayashi -- Part IV: Detection and characterization of cancer gene mutation -- 20.Mutation screeing of the TP53 gene by temporal temperature gradient gel electrophoresis. Therese Sorlie ... et al
N2  - 'Molecular Toxicology Protocols' brings together the methods most commonly used to elucidate specific molecular aspects of toxicology
ER  -