TY - BOOK AU - Kavanagh,Kevin TI - Medical mycology: cellular and molecular techniques SN - 9780470019238 (cased) : AV - QR245 .M434 2007 U1 - 616.969 KAV PY - 2007/// CY - Chichester PB - John Wiley & Sons KW - Mycoses KW - Molecular aspects KW - Laboratory manuals KW - Medical mycology KW - Biology, life sciences KW - thema KW - Medical parasitology KW - Clinical & internal medicine KW - Candidiasis KW - diagnosis KW - Laboratory Manuals KW - immunology KW - Antibodies, Monoclonal KW - diagnostic use KW - Clinical Protocols KW - Immunohistochemistry KW - methods KW - Immunologic Factors KW - Mycology N1 - Includes bibliographical references and index; 1.Diagnosis of Candia infection in tissue by immunhistochemistry -- 2.Transmission electron microscopy of pathogenic fungi -- 3.Evaluation of molecular responses and antifungal activity of phagocytes to opportunistic fungi -- 4.Determiantion of the virulence factors of candida albicans and related yeast species -- 5.Analysis of drug resistance in pathogenic fungi -- 6.Animal models for evaluation of antifungal efficacy against filamentous fungi -- 7.Proteomic analysis of pathogenic fungi -- 8.Extraction and detection of DNA and RNA from yeast -- 9.Microarrays for studying pathogenicity in candida albicans -- 10.Molecular techniques for applicaiton with asperigllus fumigatus -- 11.Promoter analysis and generation of knock-out mutants in aspergillus fumigatu 12.Microarray technology for studying the virulence of asperigillus fumigatus -- 13.Techniques and strategies for studying virulence factors in cyrptoccus neoformans -- 14.Genetic manipulation of zygomycetes N2 - In this overview of medical mycology, scientists and clinicians detail the necessary techniques for ongoing research in the field. The chapters cover a wide range of molecular, immunological and cytological techniques with illustrations, references and background material. Each chapter includes a protocol section that gives a current account of the UR - http://www.loc.gov/catdir/toc/ecip0619/2006028045.html ER -