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Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

By: Contributor(s): Material type: TextTextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2001.Edition: 3rd edDescription: 3 v. : ill. ; 28 cmISBN:
  • 9780879695774 (paper)
  • 9780879695767 (cloth)
  • 9780879695774 (Cloth)
Subject(s): DDC classification:
  • 572.8 SAM
LOC classification:
  • QH442.2 .S26 2001
Contents:
v. 1 Plasmids and Their Usefulness in Molecular Cloning -- Bacteriophage lambda and Its Vectors -- Working with Bacteriophage M13 Vectors -- Working with High-capacity Vectors -- Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis -- Preparation and Analysis of Eukaryotic Genomic DNA -- Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells. v.2 In vitro Amplification of DNA by the Polymerase Chain Reaction -- Preparation of Radiolabeled DNA and RNA Probes --- Working with Synthetic Oligonucleotide Probes -- Preparation of cDNA Libraries and Gene Identification -- DNA Sequencing -- Mutagenesis -- Screening Expression Libraries. v. 3 Expression of Cloned Genes in Escherichia coli -- Introducing Cloned Genes into Cultured Mammalian Cells -- Analysis of Gene Expression in Cultured Mammalian Cells -- Protein Interaction Technologies Appendices
Holdings
Item type Current library Call number Vol info Copy number Status Date due Barcode
Long Loan TUS: Midlands, Main Library Athlone General Lending 572.8 SAM (Browse shelf(Opens below)) v.1 1 In transit from Selfcheck to TUS: Midlands, Main Library since 21/11/2019 00213842
Long Loan TUS: Midlands, Main Library Athlone General Lending 572.8 SAM (Browse shelf(Opens below)) v.2 1 Available 00213840
Long Loan TUS: Midlands, Main Library Athlone General Lending 572.8 SAM (Browse shelf(Opens below)) v.3 1 Available 00213841

Includes bibliographical references and index.

v. 1 Plasmids and Their Usefulness in Molecular Cloning -- Bacteriophage lambda and Its Vectors -- Working with Bacteriophage M13 Vectors -- Working with High-capacity Vectors -- Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis -- Preparation and Analysis of Eukaryotic Genomic DNA -- Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells. v.2 In vitro Amplification of DNA by the Polymerase Chain Reaction -- Preparation of Radiolabeled DNA and RNA Probes --- Working with Synthetic Oligonucleotide Probes -- Preparation of cDNA Libraries and Gene Identification -- DNA Sequencing -- Mutagenesis -- Screening Expression Libraries. v. 3 Expression of Cloned Genes in Escherichia coli -- Introducing Cloned Genes into Cultured Mammalian Cells -- Analysis of Gene Expression in Cultured Mammalian Cells -- Protein Interaction Technologies Appendices

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