Laboratory exercises in microbiology, seventh edition / John P. Harley.

Includes index.

1 Microscopic Techniques 1 Bright-Field Light Microscope and Microscopic Measurement of Organisms 2 The Hanging Drop Slide and Bacterial Motility 3 Dark-Field Light Microscope 4 Phase-Contrast Light Microscope 5 Flourescence Microscope 2 Bacterial Morphology and Staining 6 Negative Staining 7 Smear Preparation and Simple Staining 8 Gram Stain 9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures 10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin) 11 Capsule Staining 12 Flagella Staining: West and Difco\'s SpotTest Methods 3 Basic Laboratory and Culture Techniques 13 Microbiological Culture Media Preparation and Sterilization 14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures 15 Spread-Plate Technique 16 The Streak-Plate Technique and Different Media 17 Pour-Plate Technique 18 Cultivation of Anaerobic Bacteria 19 Determination of Bacterial Numbers 4 Biochemical Activities of Bacteria 20 Carbohydrates I: Fermentation and B-Galactosidase Activity 21 Carbohydrates II: Triple Sugar Iron Agar Tests 22 Carbohydrates III: Starch Hydrolysis 23 Lipids: Lipid Hydrolysis 24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility 25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests 26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis 27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis 28 Proteins, Amino Acids, and Enzymes V: Catalase Activity 29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity 30 Proteins, Amino Acids, and Enzymes VII: Oxidase Test 31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity 32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test 33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination 34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction 5 Rapid Multitest Systems 35 The API 20E System 36 The Enterotube II System 6 Unknown Identification 37 Using the First Edition of Bergey\'s Manual of Systematic Bacteriology to Identify Bacteria 38 General Unknown 7 Environmental Factors Affecting Growth of Microorganisms 39 Temperature 40 pH 41 Osmotic Pressure 42 The Effects of Chemical Agents on Bacteria I: Disinfectants 43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) 44 Hand Washing, Environmental Sampling, and Microbiological Monitoring 45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods 8 Environmental and Food Microbiology 46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test 47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms 48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers 49 Enumeration of Soil Microorganisms 50 Bacterial Count of a Food Product 51 Examination of Milk for Bacteria 9 Medical Microbiology 52 Agglutination Reactions: Blood Groups 53 Isolation of Normal Microbiota from the Human Body 54 Staphylococci 55 Pneumococci 56 Streptococci 57 Neisseriae 58 Aerobic and Anaerobic Endospore-Forming Bacteria 10 Survey of Selected Eucaryotic Microorganisms 59 Fungi I: Yeasts 60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes 11 Microbial Genetics and Genomics 61 Bacterial Mutation 62 Bacterial Transformation 63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids 64 Isolation of Genomic DNA from Saccharomyces cerevisiae 65 Isolation and Purification of Genomic DNA from Escherichia coli 66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis Appendix A Dilutions with Sample Problems Appendix B Metric and English Measurement Equivalents Appendix C Transmission-Absorbance Table for Spectrophotometry Appendix D Logarithms Appendix E pH and pH Indicators Appendix F Scientific Notation Appendix G Identification Charts Appendix H Reagents, Solutions, Stains, and Tests Appendix I Culture Media Appendix J Sources and Maintenance of Microbiological Stock Culture.